GeneTex
United States (US)

PAF1 antibody

Anti-PAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107346
Anti-PAF1 antibody used in Western Blot (WB). GTX107346

Cat No. GTX107346

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Paf1 Homolog, Paf1/Rna Polymerase Ii Complex Component,F23149_1,Pd2,Paf1

Cellular Localization

Nucleus

Background

PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-PAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107346

GTX107346 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAF1(GTX107346) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAF1 antibody used in Western Blot (WB). GTX107346

GTX107346 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX107346 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169