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PAF49 antibody [GT1964]

Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115
Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115
Anti-PAF49 antibody [GT1964] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629115

Cat. No. GTX629115

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1964

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PAF49 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , A549 , H1299 , HCT116

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888097

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115

GTX629115 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX629115) diluted at 1:500.

Anti-PAF49 antibody [GT1964] used in Western Blot (WB). GTX629115

GTX629115 WB Image

PAF49 antibody [GT1964] detects PPY protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PAF49 antibody [GT1964] (GTX629115) dilution: 1:1000

Anti-PAF49 antibody [GT1964] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629115

GTX629115 ICC/IF Image

CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: CD3EAP protein stained by CD3EAP antibody (GTX629115) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations