APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , A549 , H1299 , HCT116
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888097
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49
Cellular Localization
Secreted
Database
Research Area
DATA IMAGES
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GTX629115 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX629115) diluted at 1:500.
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GTX629115 ICC/IF Image
CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: CD3EAP protein stained by CD3EAP antibody (GTX629115) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX629115 WB Image
PAF49 antibody [GT1964] detects PPY protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5 % SDS-PAGE PAF49 antibody [GT1964] (GTX629115) dilution: 1:1000
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REFERENCE
There are currently no references for PAF49 antibody [GT1964] (GTX629115). Be the first to share your publications with this product.
REVIEW
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