APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , A549 , H1299 , HCT116
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888094
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49
Cellular Localization
Nucleus , nucleolus
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Database
Research Area
DATA IMAGES
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/templates/default/js/lightbox/images/loading.gif "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
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GTX629076 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody [GT635] (GTX629076) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629076 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX629076) diluted at 1:1000.
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GTX629076 ICC/IF Image
CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: CD3EAP protein stained by CD3EAP antibody (GTX629076) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX629076 WB Image
PAF49 antibody [GT635] detects CD3EAP protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PAF49 antibody [GT635] (GTX629076) dilution: 1:1000
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REFERENCE
There are currently no references for PAF49 antibody [GT635] (GTX629076). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAF49 antibody [GT635] (GTX629076). Be the first to share your experience with this product.
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_20170511_WB_shRNA_watermark_w_23061202_478.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_20151224_WB_w_23061202_279.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_IFA_w_23061202_705.webp "Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_WB_w_23061202_718.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_20170511_WB_shRNA_watermark_w_23061202_478.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_20151224_WB_w_23061202_279.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_IFA_w_23061202_705.webp "Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_WB_w_23061202_718.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")