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PAF49 antibody

Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175
Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175
Anti-PAF49 antibody used in Western Blot (WB). GTX102175
Anti-PAF49 antibody used in IHC (Paraffin sections) (IHC-P). GTX102175

Cat No. GTX102175

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all PAF49 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49

Cellular Localization

Nucleus , nucleolus

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

Database

Research Area

DATA IMAGES

Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175

GTX102175 ICC/IF Image

PAF49 antibody detects PAF49 protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PAF49 protein stained by PAF49 antibody (GTX102175) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175

GTX102175 ICC/IF Image

PAF49 antibody detects PAF49 protein at nucleolus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAF49 protein stained by PAF49 antibody (GTX102175) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PAF49 antibody used in Western Blot (WB). GTX102175

GTX102175 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX102175) diluted at 1:1000.

Anti-PAF49 antibody used in IHC (Paraffin sections) (IHC-P). GTX102175

GTX102175 IHC-P Image

PAF49 antibody detects PAF49 protein at cytoplasm and nucleus in human ovarian cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
PAF49 antibody (GTX102175) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Daniel L et al. Proc Natl Acad Sci U S A 2018; 115 (29) : E6770-E6779 Mechanistic insights in transcription-coupled nucleotide excision repair of ribosomal DNA.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169