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PAF49 antibody

Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175
Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175
Anti-PAF49 antibody used in Western Blot (WB). GTX102175
Anti-PAF49 antibody used in IHC (Paraffin sections) (IHC-P). GTX102175

Cat No. GTX102175

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all PAF49 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240592

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49

Cellular Localization

Nucleus , nucleolus

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

Database

Research Area

DATA IMAGES

Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175

GTX102175 ICC/IF Image

PAF49 antibody detects PAF49 protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PAF49 protein stained by PAF49 antibody (GTX102175) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PAF49 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102175

GTX102175 ICC/IF Image

PAF49 antibody detects PAF49 protein at nucleolus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAF49 protein stained by PAF49 antibody (GTX102175) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PAF49 antibody used in Western Blot (WB). GTX102175

GTX102175 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX102175) diluted at 1:1000.

Anti-PAF49 antibody used in IHC (Paraffin sections) (IHC-P). GTX102175

GTX102175 IHC-P Image

PAF49 antibody detects PAF49 protein at cytoplasm and nucleus in human ovarian cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
PAF49 antibody (GTX102175) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Daniel L et al. Proc Natl Acad Sci U S A 2018; 115 (29) : E6770-E6779 Mechanistic insights in transcription-coupled nucleotide excision repair of ribosomal DNA.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169