GeneTex
United States (US)

PAG antibody [MEM-255]

PAG-antibody-MEM-255__GTX29527-1_18121411_118.jpg

Cat No. GTX29527

Host Mouse
Clonality Monoclonal
Clone Name MEM-255
Isotype IgG2a
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

WB: Use at an assay dependant dilution. Detects a band of approximately 80 kDa (predicted molecular weight: 46 kDa). Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

47 kDa. ( Note )

Positive Control

RAMOS and Jurkat cells

Specificity/Sensitivity

The antibody recognizes an epitope (aa 235-280) located in the cytoplasmic domain of Csk-binding protein (Cbp).
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human CSK Binding Protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoprotein Membrane Anchor With Glycosphingolipid Microdomains 1,Cbp,Pag,Pag1

Background

Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Database

Research Area

DATA IMAGES
PAG-antibody-MEM-255__GTX29527-1_18121411_118.jpg

GTX29527 IHC-P Image

Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255) (GTX29527). Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.

Package List Price ($)
$ 319