GeneTex
United States (US)

PAG antibody

img25174_18121411_786.jpg

Cat No. GTX14989

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 51.8 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

47 kDa. ( Note )

Positive Control

Human Ramos cell lineMouse spleen cells
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment, corresponding to amino acids 97-432 of Human PAG.

Purification

>95% by SDS-PAGE
Purified from rabbit antisera by precipitation methods.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoprotein Membrane Anchor With Glycosphingolipid Microdomains 1,Cbp,Pag,Pag1

Background

Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Database

Research Area

DATA IMAGES
img25174_18121411_786.jpg

GTX14989 IP Image

1. Frozen Raji cells lysate, immunoprecipitated with GTX24206, 2. Fresh Raji cells lysate, immunoprecipitated with GTX24206, 3. Frozen Raji cells lysate, immunoprecipitated with GTX14989, Reprobe with GTX29527. The immunoprecipitation used covalently boun

Package List Price ($)
$ 319