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PAG3 antibody [N1N2], N-term

Anti-PAG3 antibody [N1N2], N-term used in Western Blot (WB). GTX115146
Anti-PAG3 antibody [N1N2], N-term used in Western Blot (WB). GTX115146

Cat. No. GTX115146

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PAG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

Mouse brain , A431 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PAG3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620297

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 , AMAP2 , CENTB3 , DDEF2 , PAG3 , PAP , Pap-alpha , SHAG1

Cellular Localization

Cytoplasm , Golgi apparatus , Golgi stack membrane , Cell membrane

Background

This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAG3 antibody [N1N2], N-term used in Western Blot (WB). GTX115146

GTX115146 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115146 diluted at 1:1000

Anti-PAG3 antibody [N1N2], N-term used in Western Blot (WB). GTX115146

GTX115146 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Raji
7.5% SDS PAGE
GTX115146 diluted at 1:1000

REFERENCE

Application Reference

Chu H et al. Anal Chem 2022; 94 (2) : 758-767 Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169