PAI-1 antibody [1D5]

Cat. No. GTX60537

Host	Mouse
Clonality	Monoclonal
Clone Name	1D5
Isotype	IgG1
Application	WB, IHC-P, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μg ($409)

See all PAI-1 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SERPINE1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family E member 1 , PAI , PAI-1 , PAI1 , PLANH1

Cellular Localization

Secreted

Background

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Gene ID: 5054 SERPINE1
UniProt: P05121 SERPINE1

Research Area

DATA IMAGES

GTX60537 WB Image

WB analysis of human PAI-1 (AA: 194-316) recombinant protein using GTX60537 PAI-1 antibody [1D5].

GTX60537 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60537 PAI-1 antibody [1D5].

GTX60537 ELISA Image

ELISA analysis of antigen using GTX60537 PAI-1 antibody [1D5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60537 WB Image

WB analysis of HEK293 (1) and PAI-1 (AA: 194-316)-hIgGFc transfected HEK293 (2) cell lysate using GTX60537 PAI-1 antibody [1D5].

GTX60537 FACS Image

FACS analysis of NIH3T3 cells using GTX60537 PAI-1 antibody [1D5].
Green : PAI-1
Red : negative control

GTX60537 IHC-P Image

IHC-P analysis of kidney cancer tissue using GTX60537 PAI-1 antibody [1D5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 409

Qty

PAI-1 antibody [1D5]

Cat. No. GTX60537

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX60537 WB Image

GTX60537 IHC-P Image

GTX60537 ELISA Image

GTX60537 WB Image

GTX60537 FACS Image

GTX60537 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.