GeneTex
United States (US)

PAI2 antibody

GTX103194_40079_WB_M_19011506_827.jpg
GTX103194_IHC_19011506_926.jpg
GTX103194_40359_WB_19011506_849.jpg
GTX103194_42137_20151016_IP_19011506_265.jpg
GTX103194_42137_20151106_IFA_19011506_107.jpg
GTX103194_42137_20150709_WB_merged_19011506_616.jpg

Cat No. GTX103194

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A431 , JC , U87-MG

Predict Reactivity

Rabbit, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAI2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serpin Family B Member 2,Hst1201,Pai,Pai-2,Pai2,Planh2,Serpinb2

Cellular Localization

Cytoplasm , Secreted , extracellular space

Background

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

Database

DATA IMAGES
GTX103194_40079_WB_M_19011506_827.jpg

GTX103194 WB Image

Sample (30 µg of whole cell lysate)
A: JC
10% SDS PAGE
GTX103194 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103194_IHC_19011506_926.jpg

GTX103194 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PAI-2(GTX103194) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103194_40359_WB_19011506_849.jpg

GTX103194 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX103194 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103194_42137_20151016_IP_19011506_265.jpg

GTX103194 IP Image

Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 μg of PAI2 antibody (GTX103194).
Western blot analysis was performed using PAI2 antibody (GTX103194).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103194_42137_20151106_IFA_19011506_107.jpg

GTX103194 ICC/IF Image

PAI2 antibody detects PAI2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAI2 protein stained by PAI2 antibody (GTX103194) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX103194_42137_20150709_WB_merged_19011506_616.jpg

GTX103194 WB Image

PAI2 antibody detects PAI2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody (GTX103194) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169