GeneTex
United States (US)

PAI3 antibody [N3C3]

GTX106655_40135_20151015_WB_19011506_470.jpg
GTX106655_40135_20160201_IHC-P_19011506_208.jpg

Cat No. GTX106655

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HepG2 conditioned medium
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAI3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PROCI Antibody , PCI Antibody , PLANH3 Antibody , PCI-B Antibody , SERPINA5 Antibody , PAI-3 Antibody , serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Antibody

Background

The protein encoded by this gene is a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. This gene is one in a cluster of serpin genes located on the q arm of chromosome 14. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. [provided by RefSeq, Aug 2012]
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Database

DATA IMAGES
GTX106655_40135_20151015_WB_19011506_470.jpg

GTX106655 WB Image

PAI3 antibody [N3C3] detects PAI3 protein by western blot analysis. HepG2 whole cell extracts and HepG2 conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI3 antibody [N3C3] (GTX106655) diluted at 1:5000.

GTX106655_40135_20160201_IHC-P_19011506_208.jpg

GTX106655 IHC-P Image

PAI3 antibody [N3C3] detects PAI3 protein at cytoplasm in Hep G2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Hep G2 xenograft.
PAI3 antibody [N3C3] (GTX106655) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169