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PAI3 antibody

Anti-PAI3 antibody used in Western Blot (WB). GTX113795
Anti-PAI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113795
Anti-PAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113795

Cat. No. GTX113795

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PAI3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HepG2 conditioned medium

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAI3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037968

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family A member 5 , PAI-3 , PAI3 , PCI , PCI-B , PLANH3 , PROCI

Database

Research Area

DATA IMAGES

Anti-PAI3 antibody used in Western Blot (WB). GTX113795

GTX113795 WB Image

PAI3 antibody detects PAI3 protein by western blot analysis. HepG2 whole cell extracts (30 μg) and conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with PAI3 antibody (GTX113795) diluted at 1:10000.

Anti-PAI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113795

GTX113795 IHC-P Image

PAI3 antibody detects SERPINA5 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
PAI3 antibody (GTX113795) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113795

GTX113795 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PAI3(GTX113795) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169