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PAICS antibody [5B6]

Overexpression
Anti-PAICS antibody [5B6] used in Western Blot (WB). GTX83949
Anti-PAICS antibody [5B6] used in Western Blot (WB). GTX83949
Anti-PAICS antibody [5B6] used in Flow cytometry (FACS). GTX83949
Anti-PAICS antibody [5B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83949

Cat. No. GTX83949

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5B6

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all PAICS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase , ADE2 , ADE2H1 , AIRC , PAIS

Background

This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAICS antibody [5B6] used in Western Blot (WB). GTX83949

GTX83949 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83949 PAICS antibody [5B6].
Loading : 10 ug per lane
Dilution : 1:500

Anti-PAICS antibody [5B6] used in Western Blot (WB). GTX83949

GTX83949 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PAICS plasmid (Right) or empty vector (Left) for 48 hrs using GTX83949 PAICS antibody [5B6].
Loading : 5 ug per lane

Anti-PAICS antibody [5B6] used in Flow cytometry (FACS). GTX83949

GTX83949 FACS Image

FACS analysis of HEK293T cells transfected with either PAICS plasmid(Red) or empty vector control plasmid(Blue) using GTX83949 PAICS antibody [5B6].

Anti-PAICS antibody [5B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83949

GTX83949 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PAICS plasmid using GTX83949 PAICS antibody [5B6].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519