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PAK1 antibody, Internal

Anti-PAK1 antibody, Internal used in Western Blot (WB). GTX89274

Cat. No. GTX89274

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1.5μg/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89274-PEP).

Product Note

No cross-reactivity exptected with PAK3.

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NTEKQKKKPKMSDE, from the internal region of the protein sequence according to NP_002567.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 1 , IDDMSSD , PAKalpha

Cellular Localization

Cytoplasm , Cell membrane , nucleoplasm

Background

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-PAK1 antibody, Internal used in Western Blot (WB). GTX89274

GTX89274 WB Image

WB analysis of human brain (cerebral cortex) lysate using GTX89274 PAK1 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399