GeneTex

  • Country / Location Selection

United States (US)

PAK1 antibody [N2C2], Internal

Anti-PAK1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111076
Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076
Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076
Anti-PAK1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111076
Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076
Anti-PAK1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111076
Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076

Cat. No. GTX111076

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431 , BCL-1 , PC-12 , SH-SY5Y

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719641

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 1 , IDDMSSD , PAKalpha

Cellular Localization

Cytoplasm , Cell junction , focal adhesion

Background

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAK1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111076

GTX111076 IHC-P Image

PAK1 antibody [N2C2], Internal detects PAK1 protein at on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
PAK1 antibody [N2C2], Internal (GTX111076) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076

GTX111076 WB Image

Sample (30 μg of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX111076 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076

GTX111076 WB Image

PAK1 antibody [N2C2], Internal detects PAK1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PAK1 antibody [N2C2], Internal (GTX111076) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAK1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111076

GTX111076 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PAK1(GTX111076) antibody at 1:500 dilution.

Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076

GTX111076 WB Image

Sample (30 μg of whole cell lysate)
A: BCL-1
7.5% SDS PAGE
GTX111076 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAK1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111076

GTX111076 IHC-P Image

PAK1 antibody [N2C2], Internal detects PAK1 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
PAK1 antibody [N2C2], Internal (GTX111076) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAK1 antibody [N2C2], Internal used in Western Blot (WB). GTX111076

GTX111076 WB Image

PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody (GTX111076) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen MJ et al. Sci Rep 2016; 6 : 34933 PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PAK1 antibody [N2C2], Internal (GTX111076). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169