GeneTex
United States (US)

PAK1 antibody

GTX103797_40114_WB_M_19011506_111.jpg
GTX103797_40114_IFA_19011506_161.jpg
GTX103797_40114_20150820_WB_19011506_232.jpg
GTX103797_40114_WB_R_19011506_667.jpg
GTX103797_40114_IHC_19011506_304.jpg
GTX103797_40114_WB_19011506_565.jpg

Cat No. GTX103797

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431 , HeLa , BCL-1 , PC-12 , SH-SY5Y

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P21 (Rac1) Activated Kinase 1,Pakalpha,Pak1

Cellular Localization

Cytoplasm , Cell junction , focal adhesion

Background

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103797_40114_WB_M_19011506_111.jpg

GTX103797 WB Image

PAK1 antibody detects PAK1 protein by western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
PAK1 antibody (GTX103797) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103797_40114_IFA_19011506_161.jpg

GTX103797 ICC/IF Image

PAK1 antibody detects PAK1 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: PAK1 protein stained by PAK1 antibody (GTX103797) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX103797_40114_20150820_WB_19011506_232.jpg

GTX103797 WB Image

PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody (GTX103797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103797_40114_WB_R_19011506_667.jpg

GTX103797 WB Image

PAK1 antibody detects PAK1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PAK1 antibody (GTX103797) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103797_40114_IHC_19011506_304.jpg

GTX103797 IHC-P Image

PAK1 antibody detects PAK1 protein on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
PAK1 antibody (GTX103797) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103797_40114_WB_19011506_565.jpg

GTX103797 WB Image

Sample (30 µg of whole cell lysate)
A: A431
B: HeLa
7.5% SDS PAGE
GTX103797 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf