APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
1:50-1:100 |
Not tested in other applications.
Product Note
This antibody is raised against PAK1 phosphorylated at Thr423. Based on sequence homology, it is predicted to react with PAK2 and PAK3 when phosphorylated at the corresponding residues - Thr402/Thr436.
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V) derived from human PAK1 / PAK2 / PAK3.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
p21 (RAC1) activated kinase 1 , p21 (RAC1) activated kinase 2 , p21 (RAC1) activated kinase 3 , IDDMSSD , PAKalpha , PAK65 , PAKgamma , ARA , MRX30 , MRX47 , OPHN3 , PAK-3 , PAK3beta , bPAK , beta-PAK
Cellular Localization
Cytoplasm,Cell membrane,nucleoplasm
Background
The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017]
Database
Research Area
DATA IMAGES
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GTX50867 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX50867 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody.
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GTX50867 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50867 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
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REFERENCE
There are currently no references for PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody (GTX50867). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody (GTX50867). Be the first to share your experience with this product.