*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody is raised against PAK1 phosphorylated at Thr423. Based on sequence homology, it is predicted to react with PAK2 and PAK3 when phosphorylated at the corresponding residues - Thr402/Thr436.
Mouse, Rat(>80% identity)
PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide sequence around phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V) derived from human PAK1 / PAK2 / PAK3.
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
p21 (RAC1) activated kinase 1 , p21 (RAC1) activated kinase 2 , p21 (RAC1) activated kinase 3 , IDDMSSD , PAKalpha , PAK65 , PAKgamma , ARA , MRX30 , MRX47 , OPHN3 , PAK-3 , PAK3beta , bPAK , beta-PAK
The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017]
GTX50867 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX50867 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody.
GTX50867 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50867 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide