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PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50867
Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in IHC (Paraffin sections) (IHC-P). GTX50867

Cat No. GTX50867

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Specificity/Sensitivity

This antibody is raised against PAK1 phosphorylated at Thr423. Based on sequence homology, it is predicted to react with PAK2 and PAK3 when phosphorylated at the corresponding residues - Thr402/Thr436.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V) derived from human PAK1 / PAK2 / PAK3.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

p21 (RAC1) activated kinase 1 , p21 (RAC1) activated kinase 2 , p21 (RAC1) activated kinase 3 , IDDMSSD , PAKalpha , PAK65 , PAKgamma , ARA , MRX30 , MRX47 , OPHN3 , PAK-3 , PAK3beta , bPAK , beta-PAK

Cellular Localization

Cytoplasm,Cell membrane,nucleoplasm

Background

The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50867

GTX50867 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50867 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody.

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in IHC (Paraffin sections) (IHC-P). GTX50867

GTX50867 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50867 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody (GTX50867). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody (GTX50867). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf