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PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Western Blot (WB). GTX82619

Cat. No. GTX82619

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Rat
Package
100 μl ($499)
See all PAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

This antibody is raised against PAK1 phosphorylated at Thr423. Based on sequence homology, it is predicted to react with PAK2 and PAK3 when phosphorylated at the corresponding residues - Thr402/Thr436.

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 1

Cellular Localization

Cytoplasm , Cell membrane , nucleoplasm

Background

serine/threonine protein kinase; binds and forms complex specifically with activated (GTP-bound) p21, leading to inhibiting p21 GTPase activity [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody used in Western Blot (WB). GTX82619

GTX82619 WB Image

WB analysis of rat hippocampal tissue lysate using GTX82619 PAK1 ( phospho Thr423 ) / PAK2 ( phospho Thr402 ) / PAK3 ( phospho Thr436 ) antibody.
Lane 1 : primary antibody
Lane 2 : treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 499