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PAK2 antibody [3B5]

Anti-PAK2 antibody [3B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83373
Anti-PAK2 antibody [3B5] used in Western Blot (WB). GTX83373
Anti-PAK2 antibody [3B5] used in IHC (Paraffin sections) (IHC-P). GTX83373

Cat. No. GTX83373

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3B5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Monkey
Package
100 μl ($439)
See all PAK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of PAK2(AA : 4-223) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 2 , PAK65 , PAKgamma

Background

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PAK2 antibody [3B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83373

GTX83373 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83373 PAK2 antibody [3B5].
Green : PAK2
Blue: DRAQ5 fluorescent DNA dye

Anti-PAK2 antibody [3B5] used in Western Blot (WB). GTX83373

GTX83373 WB Image

WB analysis of HeLa (1), Jurkat (2), A549 (3), HEK293 (4) and K562 (5) cell lysate using GTX83373 PAK2 antibody [3B5].

Anti-PAK2 antibody [3B5] used in IHC (Paraffin sections) (IHC-P). GTX83373

GTX83373 IHC-P Image

IHC-P analysis of human lung cancer (left) and gastric cancer (right) using GTX83373 PAK2 antibody [3B5].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 439