PAK2 antibody [GT1259]

Cat. No. GTX02856

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1259
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Package	100 μl ($419)

See all PAK2 products

Datasheet

View product citations for antibody GTX02856 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
ICC/IF	1:50 - 1:200

Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PAK2

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 2 , PAK65 , PAKgamma

Background

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5062 PAK2
UniProt: Q13177 PAK2

Research Area

DATA IMAGES

GTX02856 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAK2 antibody [GT1259] (GTX02856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02856 WB Image

GTX02856 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX02856 PAK2 antibody [GT1259].
Dilution : 1:100
Blue : DAPI for nuclear staining.

GTX02856 WB Image

WB analysis of various samples using GTX02856 PAK2 antibody [GT1259].
Dilution : 1:1000
Loading : 25μg

GTX02856 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX02856 PAK2 antibody [GT1259].
Dilution : 1:100
Blue : DAPI for nuclear staining.

GTX02856 ICC/IF Image

ICC/IF analysis of C6 cells using GTX02856 PAK2 antibody [GT1259].
Dilution : 1:100
Blue : DAPI for nuclear staining.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 419

Qty

PAK2 antibody [GT1259]

Cat. No. GTX02856

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX02856 WB Image

GTX02856 WB Image

GTX02856 ICC/IF Image

GTX02856 WB Image

GTX02856 ICC/IF Image

GTX02856 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.