Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX104122 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
7.5% SDS PAGE
GTX109991 diluted at 1:1000

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK3 antibody (GTX109991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.