APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500~1:1000 |
| 1:50~1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human PAK3 (121-170).
Purification
Purified by antigen-affinity chromatography. Non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ARA , MRX30 , MRX47 , OPHN3 , PAK3 , PAK3beta , bPAK , betaPAK , p21 (RAC1) activated kinase 3 , p21 protein (Cdc42/Rac)-activated kinase 3
Cellular Localization
Cytoplasm
Background
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX86634 IHC-P Image
IHC-P analysis of human brain tissue using GTX86634 PAK3 (phospho Ser154) antibody. The picture on the right is blocked with the synthesized peptide.
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GTX86634 WB Image
WB analysis of rat heart lysates using GTX86634 PAK3 (phospho Ser154) antibody. The lane on the right is blocked with the synthesized peptide.
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REFERENCE
There are currently no references for PAK3 (phospho Ser154) antibody (GTX86634). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAK3 (phospho Ser154) antibody (GTX86634). Be the first to share your experience with this product.
