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PAK4 antibody

Anti-PAK4 antibody used in Western Blot (WB). GTX31673
Anti-PAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31673
Anti-PAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31673

Cat. No. GTX31673

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($419)
See all PAK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

SW480 Cell Lysate(GTX23957) , Human Colon Tissue Lysate(GTX27928)

Product Note

PAK4 antibody is predicted to not cross-react with other PAK family proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PAK4 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4.The immunogen is located within amino acids 210 - 260 of PAK4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 4

Cellular Localization

Cytoplasm

Background

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PAK4 antibody used in Western Blot (WB). GTX31673

GTX31673 WB Image

WB analysis of SW480 cell lysate using GTX31673 PAK4 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31673

GTX31673 IHC-P Image

IHC-P analysis of human colon tissue using GTX31673 PAK4 antibody.
Working concentration : 20 μg/ml

Anti-PAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31673

GTX31673 IHC-P Image

IHC-P analysis of human colon tissue using GTX31673 PAK4 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419