GeneTex
United States (US)

PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody

GTX32289_20190131_ICCIF_19013102_760.jpg
GTX32289_20190131_WB_19013102_835.jpg

Cat No. GTX32289

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Specificity/Sensitivity

This antibody is raised against PAK4 phosphorylated at Ser474. Based on sequence homology, it is predicted to react with PAK5 and PAK6 when phosphorylated at the corresponding residues - Ser602/Ser560.
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of PAK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P21 (Rac1) Activated Kinase 4,Pak4

Cellular Localization

Cytoplasm

Background

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32289_20190131_ICCIF_19013102_760.jpg

GTX32289 ICC/IF Image

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32289 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32289_20190131_WB_19013102_835.jpg

GTX32289 WB Image

WB analysis of EGF-treated HEK293T (A), EGF-treated Raw264.7 (B), EGF-treated PC12 (C) whole cell lysates using GTX32289 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody.

REFERENCE
Package List Price ($)
$ 319