GeneTex
United States (US)

PAK6 antibody

GTX107861_40016_IHC_19011507_645.jpg
GTX107861_40016_WB_B_19011507_522.jpg
GTX107861_40016_IP_19011507_841.jpg

Cat No. GTX107861

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

293T transfected recombinant PAK6 protein

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAK6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P21 (Rac1) Activated Kinase 6,Pak5,Pak6

Background

This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107861_40016_IHC_19011507_645.jpg

GTX107861 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PAK6(GTX107861) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107861_40016_WB_B_19011507_522.jpg

GTX107861 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: PAK6 transfected 293T lysates
7.5% SDS PAGE
GTX107861 diluted at 1:5000

GTX107861_40016_IP_19011507_841.jpg

GTX107861 IP Image

PAK6 antibody immunoprecipitates PAK6 protein in IP experiments.
IP samples: PC-3 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PAK6 protein by 4 μg PAK6 antibody (GTX107861)
7.5 % SDS-PAGE
The immunoprecipitated PAK6 protein was detected by PAK6 antibody (GTX107861) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169