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PAK6 antibody

Anti-PAK6 antibody used in Western Blot (WB). GTX127915
Anti-PAK6 antibody used in IHC (Paraffin sections) (IHC-P). GTX127915
Anti-PAK6 antibody used in Immunoprecipitation (IP). GTX127915
Anti-PAK6 antibody used in Western Blot (WB). GTX127915
Anti-PAK6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127915

Cat. No. GTX127915

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PAK6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PAK6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2687660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 6 , PAK5

Background

This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAK6 antibody used in Western Blot (WB). GTX127915

GTX127915 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK6 antibody (GTX127915) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAK6 antibody used in IHC (Paraffin sections) (IHC-P). GTX127915

GTX127915 IHC-P Image

PAK6 antibody detects PAK6 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
PAK6 antibody (GTX127915) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAK6 antibody used in Immunoprecipitation (IP). GTX127915

GTX127915 IP Image

PAK6 antibody immunoprecipitates PAK6 protein in IP experiments.
IP samples: PC-3 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PAK6 protein by 4 μg PAK6 antibody (GTX127915)
7.5 % SDS-PAGE
The immunoprecipitated PAK6 protein was detected by PAK6 antibody (GTX127915) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-PAK6 antibody used in Western Blot (WB). GTX127915

GTX127915 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK6 antibody (GTX127915) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAK6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127915

GTX127915 ICC/IF Image

The data was published in the journal Dev Cell in 2017.PMID: 28486133

REFERENCE

Application Reference

Foxall E et al. Cell Rep 2019; 29 (11) : 3385-3393.e6 PAK4 Kinase Activity Plays a Crucial Role in the Podosome Ring of Myeloid Cells.
Durkin CH et al. Dev Cell 2017; 41 (3) : 315-329.e7 RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169