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PANK1 antibody [N3C3]

Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920
Anti-PANK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105920
Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920
Anti-PANK1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105920

Cat No. GTX105920

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PANK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pantothenate Kinase 1,Pank,Pank1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. Alternative splicing has been observed at this locus and three variants, each encoding a distinct isoform, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920

GTX105920 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX105920 diluted at 1:1000

Anti-PANK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105920

GTX105920 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using PANK1(GTX105920) antibody at 1:500 dilution.

Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920

GTX105920 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105920 diluted at 1:5000

Anti-PANK1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105920

GTX105920 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PANK1(GTX105920) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169