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PANK1 antibody [N3C3]

Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920
Anti-PANK1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105920
Anti-PANK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105920
Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920

Cat. No. GTX105920

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PANK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

Mouse liver , HepG2

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PANK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951103

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pantothenate kinase 1 , PANK

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. Alternative splicing has been observed at this locus and three variants, each encoding a distinct isoform, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920

GTX105920 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX105920 diluted at 1:1000

Anti-PANK1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105920

GTX105920 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PANK1(GTX105920) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PANK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105920

GTX105920 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using PANK1(GTX105920) antibody at 1:500 dilution.

Anti-PANK1 antibody [N3C3] used in Western Blot (WB). GTX105920

GTX105920 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105920 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169