APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture : GTX10192, Detection : GTX10195. Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A).
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific to PAPP A subunit of the heterotetrameric complex of PAPP-A (htPAPP-A). It can be used to detect htPAPP-A (when paired with another antibody specific for proMBP) or PAPP-A dimer (when paired with another antibody specific for PAPP A subunit)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pappalysin 1 , ASBABP2 , DIPLA1 , IGFBP-4ase , PAPA , PAPP-A , PAPPA1
Cellular Localization
Secreted
Background
This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-PAPP A antibody [10H9] used in ELISA (ELISA). GTX10195](/templates/default/js/lightbox/images/loading.gif "Anti-PAPP A antibody [10H9] used in ELISA (ELISA). GTX10195")
|
GTX10195 ELISA Image
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] (GTX10192) biotinylated; Detection: PAPP A antibody [10H9] (GTX10195) labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.
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REFERENCE
There are currently no references for PAPP A antibody [10H9] (GTX10195). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAPP A antibody [10H9] (GTX10195). Be the first to share your experience with this product.
![Anti-PAPP A antibody [10H9] used in ELISA (ELISA). GTX10195](/upload/website/prouct_img/small/GTX10195/PAPP-A-antibody-10H9_ELISA_GTX10195-1_w_23060100_599.webp "Anti-PAPP A antibody [10H9] used in ELISA (ELISA). GTX10195")
![Anti-PAPP A antibody [10H9] used in ELISA (ELISA). GTX10195](/upload/website/prouct_img/normal/GTX10195/PAPP-A-antibody-10H9_ELISA_GTX10195-1_w_23060100_599.webp "Anti-PAPP A antibody [10H9] used in ELISA (ELISA). GTX10195")