APPLICATION
Application Note
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 200 kDa under reducing conditions. (predicted molecular weight: 195 kDa). Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Antigen Species
Human
Immunogen
Purified human PAPPA antigen
Purification
Protein A purified
Purified by chromatography on protein A. Purity tested by electrophoresis
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1, PAPP A, IGFBP 4ase, PAPPA, IGFBP4ase
Background
Pregnancy-associated plasma protein A (PAPPA) is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses.
Database
DATA IMAGES
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GTX10196 WB Image
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] (GTX10196); Lane 2: PAPP A antibody [3C8] (GTX10194); Lane 3: PAPP A antibody [5H9] (GTX10191); Lane 4: PAPP A antibody [11E4] (GTX14597); Lane 5: PAPP A antibody [10A5] (GTX10193); Lane 6: PAPP A antibody [3C8] (GTX10194); Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] (GTX10193) and PAPP A antibody [3C8] (GTX10194)
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