GeneTex
United States (US)

PAPP A antibody [3C8]

PAPP-A-antibody-3C8_Western blot_GTX10194-1_19011720_493.jpg
PAPP-A-antibody-3C8_ELISA_GTX10194-1_19011506_133.jpg

Cat No. GTX10194

Host Mouse
Clonality Monoclonal
Clone Name 3C8
Isotype IgG2a
Application WB, ELISA, IA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
IA Assay dependent
Not tested in other applications.

Calculated MW

73.52 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific to PAPP A subunit of the heterotetrameric complex of PAPP-A (htPAPP-A). It can be used to detect htPAPP-A (when paired with another antibody specific for proMBP) or PAPP-A dimer (when paired with another antibody specific for PAPP A subunit)
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pappalysin 1,Asbabp2,Dipla1,Igfbp-4Ase,Papa,Papp-A,Pappa1,Pappa

Background

This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
PAPP-A-antibody-3C8_Western blot_GTX10194-1_19011720_493.jpg

GTX10194 WB Image

Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] (GTX10196); Lane 2: PAPP A antibody [3C8] (GTX10194); Lane 3: PAPP A antibody [5H9] (GTX10191); Lane 4: PAPP A antibody [11E4] (GTX14597); Lane 5: PAPP A antibody [10A5] (GTX10193); Lane 6: PAPP A antibody [3C8] (GTX10194); Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] (GTX10193) and PAPP A antibody [3C8] (GTX10194)

PAPP-A-antibody-3C8_ELISA_GTX10194-1_19011506_133.jpg

GTX10194 ELISA Image

Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] (GTX10192) biotinylated; Detection: PAPP A antibody [3C8] (GTX10194) labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.

Package List Price ($)
$ 239