*Optimal dilutions/concentrations should be determined by the researcher.
Capture : GTX10192, Detection : GTX10194. Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A).
Not tested in other applications.
This antibody is specific to PAPP A subunit of the heterotetrameric complex of PAPP-A (htPAPP-A). It can be used to detect htPAPP-A (when paired with another antibody specific for proMBP) or PAPP-A dimer (when paired with another antibody specific for PAPP A subunit)
PBS pH 7.4, 0.09 % sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.
Batch dependent (Please refer to the vial label for the specific concentration.)
Purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Pappalysin 1 , Asbabp2 , Dipla1 , Igfbp-4Ase , Papa , Papp-A , Pappa1 , Pappa
This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. [provided by RefSeq, Jul 2008]
GTX10194 WB Image
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] (GTX10196); Lane 2: PAPP A antibody [3C8] (GTX10194); Lane 3: PAPP A antibody [5H9] (GTX10191); Lane 4: PAPP A antibody [11E4] (GTX14597); Lane 5: PAPP A antibody [10A5] (GTX10193); Lane 6: PAPP A antibody [3C8] (GTX10194); Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] (GTX10193) and PAPP A antibody [3C8] (GTX10194)
GTX10194 ELISA Image
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] (GTX10192) biotinylated; Detection: PAPP A antibody [3C8] (GTX10194) labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.