GeneTex
United States (US)

PAPP A antibody [4G11]

PAPP-A-antibody-4G11_ELISA_GTX10192-1_19011506_466.jpg
PAPP-A-antibody-4G11_ELISA_GTX10192-2_19011506_338.jpg

Cat No. GTX10192

Host Mouse
Clonality Monoclonal
Clone Name 4G11
Isotype IgG2a
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. Can be paired for ELISA with GTX10194 Mouse monoclonal [3C8] to PAPPA and GTX10195 Mouse monoclonal [10H9] to PAPPA . WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. GTX10192 recognizes heterotetrameric complex in Western Blotting after SDS-electrophoresis in non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

181 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human PAPPA antigen

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1, PAPP A, IGFBP 4ase, PAPPA, IGFBP4ase

Background

Pregnancy-Assosiated Plasma Protein A (PAPPA) is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses.

Database

DATA IMAGES
PAPP-A-antibody-4G11_ELISA_GTX10192-1_19011506_466.jpg

GTX10192 ELISA Image

Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] (GTX10192) biotinylated; Detection: PAPP A antibody [3C8] (GTX10194) labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.

PAPP-A-antibody-4G11_ELISA_GTX10192-2_19011506_338.jpg

GTX10192 ELISA Image

Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] (GTX10192) biotinylated; Detection: PAPP A antibody [10H9] (GTX10195) labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.

Package List Price ($)
$ 229