APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2 , mouse brain
Predict Reactivity
Rat, Zebrafish, Rabbit, Bovine, Guinea pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.94 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PAPSS1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
3'-phosphoadenosine 5'-phosphosulfate synthase 1 , ATPSK1 , PAPSS , SK1
Background
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]
Database
DATA IMAGES
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GTX103608 WB Image
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX103608 diluted at 1:1000
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GTX103608 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PAPSS1(GTX103608) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103608 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using PAPSS1(GTX103608) antibody at 1:200 dilution.
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GTX103608 WB Image
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX103608 diluted at 1:500
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REFERENCE
There are currently no references for PAPSS1 antibody [C1C3] (GTX103608). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAPSS1 antibody [C1C3] (GTX103608). Be the first to share your experience with this product.