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PAPSS1 antibody [C1C3]

Anti-PAPSS1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103608
Anti-PAPSS1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX103608
Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608
Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608

Cat. No. GTX103608

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PAPSS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Mouse brain , HepG2

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Guinea pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAPSS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037609

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3'-phosphoadenosine 5'-phosphosulfate synthase 1 , ATPSK1 , PAPSS , SK1

Background

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PAPSS1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103608

GTX103608 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PAPSS1(GTX103608) antibody at 1:200 dilution.

Anti-PAPSS1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX103608

GTX103608 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PAPSS1(GTX103608) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608

GTX103608 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX103608 diluted at 1:1000

Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608

GTX103608 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX103608 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169