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PAPSS1 antibody [C1C3]

Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608
Anti-PAPSS1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX103608
Anti-PAPSS1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103608
Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608

Cat No. GTX103608

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all PAPSS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Guinea pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAPSS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

3'-phosphoadenosine 5'-phosphosulfate synthase 1 , ATPSK1 , PAPSS , SK1

Background

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]

Database

DATA IMAGES

Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608

GTX103608 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX103608 diluted at 1:1000

Anti-PAPSS1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX103608

GTX103608 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PAPSS1(GTX103608) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAPSS1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103608

GTX103608 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PAPSS1(GTX103608) antibody at 1:200 dilution.

Anti-PAPSS1 antibody [C1C3] used in Western Blot (WB). GTX103608

GTX103608 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX103608 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169