APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
3T3 (NIH) Cell Lysate(GTX27179) , 3T3/BALB Cell Slide
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
PARL antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PARL.The immunogen is located within amino acids 40 - 90 of PARL.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
presenilin associated rhomboid like , PRO2207 , PSARL , PSARL1 , PSENIP2 , RHBDS1
Cellular Localization
Mitochondrion inner membrane
Background
PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL’s association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer’s disease through its associations with presenilin-1 and -2. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Multiple isoforms of PARL are known to exist.
Database
Research Area
DATA IMAGES
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GTX85382 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX85382 PARL antibody.
Working concentration : 2.5 μg/ml
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GTX85382 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX85382 PARL antibody.
Working concentration : 2.5 μg/ml
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GTX85382 WB Image
WB analysis of 3T3 cell lysate using GTX85382 PARL antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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REFERENCE
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REVIEW
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