*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
NIH-3T3 , BCL-1
Knockdown/Knockout validation was supported by references (PMID:26990993)
Rat, Bovine(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant protein encompassing a sequence within the center region of human PARN. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DAN antibody, PARN antibody, deadenylation nuclease antibody, deadenylating nuclease antibody, poly(A)-specific ribonuclease (deadenylation nuclease) antibody, poly(A)-specific ribonuclease PARN antibody, polyadenylate-specific ribonuclease antibody
Nucleus , Cytoplasm , nucleolus
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX115020 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX115020 diluted at 1:2000