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PARP antibody [10H]

Anti-PARP antibody [10H] used in Immunohistochemistry (IHC). GTX14459

Cat. No. GTX14459

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10H

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-Fr, ELISA, IHC

Reactivity

 Human, Bovine
Package
100 μg ($399)
See all PARP products

APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 5 - 20 μg/ml. WB: Use at a concentration of 2 μg/ml, if using ECL or 10 μg/ml, if using colorimetric methods. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

113 kDa. ( Note )

Positive Control

Rat liver induced for PADPR synthesis by injection with diethylnitrosamine.

Product Note

This antibody reacts with PADPR synthesized by a variety of poly(ADP- ribose) polymerases (PARP)-related enzymes including PARP1, 2, 3, tankyrase, vPARP, sPARP and others.The antibody does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA.This antibody crossreacts to bovine serum albumin due to its use as a carrier for the immunogen.

PROPERTIES

Form

Liquid

Buffer

20mM Tris, 150mM Sodium Chloride pH7.4, 1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

PADPR mixed with methylated bovine serum albumin.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_372767

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [10H] used in Immunohistochemistry (IHC). GTX14459

GTX14459 IHC Image

Immunohistochemistry of livers treated with diethylnitrosamine (200 mg/kg) andstained with GTX14459 diluted 1/100.After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction.

REFERENCE

Application Reference

C?saire M et al. J Bone Oncol 2019; 17 : 100246 Sensitization of chondrosarcoma cells with PARP inhibitor and high-LET radiation.
Application : WB
Reactivity : Human
Gottipati P et al. Cancer Res 2010; 70 (13) : 5389-98 Poly(ADP-ribose) polymerase is hyperactivated in homologous recombination-defective cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399