GeneTex
United States (US)

PARP antibody [10H]

PARP-antibody-10H-GTX14459-IHC-1_18121411_151.jpg

Cat No. GTX14459

Host Mouse
Clonality Monoclonal
Clone Name 10H
Isotype IgG3
Application WB, ICC/IF, IHC-Fr, ELISA, IHC
Reactivity Human, Bovine, Cow
APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 5 - 20 μg/ml. WB: Use at a concentration of 2 μg/ml, if using ECL or 10 μg/ml, if using colorimetric methods. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Rat liver induced for PADPR synthesis by injection with diethylnitrosamine.

Specificity/Sensitivity

This antibody reacts with PADPR synthesized by a variety of poly(ADP- ribose) polymerases (PARP)-related enzymes including PARP1, 2, 3, tankyrase, vPARP, sPARP and others.The antibody does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA.This antibody crossreacts to bovine serum albumin due to its use as a carrier for the immunogen.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide; Constituents: 1% BSA, 20mM Tris, 150mM Sodium Chloride. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

PADPR mixed with methylated bovine serum albumin.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).

Research Area

DATA IMAGES
PARP-antibody-10H-GTX14459-IHC-1_18121411_151.jpg

GTX14459 IHC Image

Immunohistochemistry of livers treated with diethylnitrosamine (200 mg/kg) andstained with GTX14459 diluted 1/100.After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction.

REFERENCE
Package List Price ($)
$ 319