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PARP antibody [7A10]

Anti-PARP antibody [7A10] used in Flow cytometry (FACS). GTX83005
Anti-PARP antibody [7A10] used in Western Blot (WB). GTX83005

Cat. No. GTX83005

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7A10

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide of human PARP, conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [7A10] used in Flow cytometry (FACS). GTX83005

GTX83005 FACS Image

FACS analysis of Jurkat cells using GTX83005 PARP antibody [7A10].
Green : PARP
Purple : negative control

Anti-PARP antibody [7A10] used in Western Blot (WB). GTX83005

GTX83005 WB Image

WB analysis of Jurkat (1), K562 (2), HeLa (3), Raji (4),THP-1 (5) and SW620 (6) cell lysate using GTX83005 PARP antibody [7A10].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399