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PARP antibody [A6.4.12]

Anti-PARP antibody [A6.4.12] used in Western Blot (WB). GTX75098
Anti-PARP antibody [A6.4.12] used in Western Blot (WB). GTX75098

Cat. No. GTX75098

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 A6.4.12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

 Human, Mouse, Rat, Drosophila, Hamster, Xenopus
Package
100 μg ($449)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/1,000-1/5,000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PARP-1

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377789

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [A6.4.12] used in Western Blot (WB). GTX75098

GTX75098 WB Image

WB analysis of HeLa nuclear extract using GTX75098 PARP antibody [A6.4.12].

Anti-PARP antibody [A6.4.12] used in Western Blot (WB). GTX75098

GTX75098 WB Image

WB analysis of HEK293 cell lysate using GTX75098 PARP antibody [A6.4.12].

REFERENCE

Application Reference

Zhou Y et al. Nucleic Acids Res 2014; 42 (3) : e19 Quantitation of DNA double-strand break resection intermediates in human cells.
Application : WB
Reactivity : Human
Zhou Y et al. J Biol Chem 2013; 288 (52) : 37112-25 DNA-dependent protein kinase regulates DNA end resection in concert with Mre11-Rad50-Nbs1 (MRN) and ataxia telangiectasia-mutated (ATM).
Application : WB
Reactivity : Human
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1) : 104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
Sinanoglu O et al. Urology 2012; 80 (6) : 1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449