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PARP antibody [GT1176]

Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509
Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509
Anti-PARP antibody [GT1176] used in IHC (Paraffin sections) (IHC-P). GTX09509
Anti-PARP antibody [GT1176] used in IHC (Paraffin sections) (IHC-P). GTX09509
Anti-PARP antibody [GT1176] used in IHC (Paraffin sections) (IHC-P). GTX09509
Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509
Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509

Cat No. GTX09509

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1176

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.3) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifμge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan PARP

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509

GTX09509 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT1176] (GTX09509) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509

GTX09509 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT1176] (GTX09509) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PARP antibody [GT1176] used in IHC (Paraffin sections) (IHC-P). GTX09509

GTX09509 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX09509 PARP antibody [GT1176].
Dlution : 1:100

Anti-PARP antibody [GT1176] used in IHC (Paraffin sections) (IHC-P). GTX09509

GTX09509 IHC-P Image

IHC-P analysis of hmuan appendix tissue section using GTX09509 PARP antibody [GT1176].
Dlution : 1:100

Anti-PARP antibody [GT1176] used in IHC (Paraffin sections) (IHC-P). GTX09509

GTX09509 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX09509 PARP antibody [GT1176].
Dlution : 1:100

Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509

GTX09509 WB Image

WB analysis of Wild-type (WT) and PARP knockout (KO) HeLa cell extracts using GTX09509 PARP antibody [GT1176].
Dilution : 1:1000
Loading : 25μg

Anti-PARP antibody [GT1176] used in Western Blot (WB). GTX09509

GTX09509 WB Image

WB analysis of Jurkat cells were treated with/without Staurosporine (1μM) at RT for 3 hrs using GTX09509 PARP antibody [GT1176].
Dilution : 1:1000
Loading : 25μg

REFERENCE

There are currently no references for PARP antibody [GT1176] (GTX09509). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PARP antibody [GT1176] (GTX09509). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369