GeneTex

  • Country / Location Selection

United States (US)

PARP antibody [GT6212]

KO/KD Orthogonal Overexpression IP/MS
Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836
Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836
Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836
Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836
Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836
Anti-PARP antibody [GT6212] used in IHC (Paraffin sections) (IHC-P). GTX628836
Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836
Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

Cat. No. GTX628836

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT6212

Isotype

 IgG2b

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , HCT116 (30μM Cisplatin treatment for 24hr) , PC-12 , Rat2

Product Note

This antibody is specific for human PARP1 protein, and it does not cross react with human PARP2 and PARP3 protein.
IP/MS validation was supported by references (PMID:30377422)

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888062

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

GTX628836 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000.

Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

GTX628836 WB Image

Orthogonal

Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

GTX628836 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

GTX628836 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

GTX628836 WB Image

Orthogonal

PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated)
B. 30 μg HCT116 whole cell lysate/extract (30μM Cisplatin treatment for 24hr)
7.5 % SDS-PAGE
PARP1 antibody [GT6212] (GTX628836) dilution: 1:1000

Anti-PARP antibody [GT6212] used in IHC (Paraffin sections) (IHC-P). GTX628836

GTX628836 IHC-P Image

PARP antibody [GT6212] detects PARP protein at nucleus in human Hep G2 by immunohistochemical analysis.
Sample: Paraffin-embedded human Hep G2.
PARP antibody [GT6212] (GTX628836) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

GTX628836 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PARP antibody [GT6212] used in Western Blot (WB). GTX628836

GTX628836 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] (GTX628836) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Raineri A et al. Molecules. 2021; 26 (16) : 5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.
Application : WB
Reactivity : Mouse
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Xue P et al. PLoS One 2014; 9 (5) : e97360 Regulation of ABCC6 Trafficking and Stability by a Conserved C-terminal PDZ-Like Sequence.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PARP antibody [GT6212] (GTX628836). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations