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PARP antibody [GT982]

KO/KD Orthogonal Overexpression IP/MS
Anti-PARP antibody [GT982] used in Western Blot (WB). GTX632388
Anti-PARP antibody [GT982] used in Immunoprecipitation (IP). GTX632388
Anti-PARP antibody [GT982] used in Western Blot (WB). GTX632388
Anti-PARP antibody [GT982] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632388
Anti-PARP antibody [GT982] used in Western Blot (WB). GTX632388

Cat. No. GTX632388

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT982

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

HeLa (30uM cisplatin treatment for 24hr) , 293T

Product Note

This antibody is specific for human PARP1 protein, and it does not cross react with human PARP2 and PARP3 protein.
IP/MS validation was supported by references (PMID:30377429)

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888311

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [GT982] used in Western Blot (WB). GTX632388

GTX632388 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:1000.

Anti-PARP antibody [GT982] used in Immunoprecipitation (IP). GTX632388

GTX632388 IP Image

Immunoprecipitation of PARP protein from 293T whole cell extracts using 5 μg of PARP antibody (GTX632388).
Western blot analysis was performed using PARP antibody (GTX632388).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-PARP antibody [GT982] used in Western Blot (WB). GTX632388

GTX632388 WB Image

Orthogonal

PARP1 antibody detects PARP1 protein by western blot analysis. Un-treated (-) and treated (+, 30uM cisplatin treatment for 24hr) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody (GTX632388) at a dilution of 1:1000.

Anti-PARP antibody [GT982] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632388

GTX632388 ICC/IF Image

PARP1 antibody [GT982] detects PARP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP1 protein stained by PARP1 antibody [GT982] (GTX632388) diluted at 1:400.
Red: Phalloidin, a F-actin marker, diluted at 1:200.
Anti-PARP antibody [GT982] used in Western Blot (WB). GTX632388

GTX632388 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:50000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations