*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , HeLa , HeLa (30uM cisplatin treatment for 24hr)
IP/MS validation was supported by references (PMID:30377429)
Mouse, Rat, Bovine(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.65 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
GTX632388 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] (GTX632388) diluted at 1:1000.
GTX632388 ICC/IF Image
PARP1 antibody [GT982] detects PARP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP1 protein stained by PARP1 antibody [GT982] (GTX632388) diluted at 1:400.
Red: Phalloidin, a F-actin marker, diluted at 1:200.
GTX632388 WB Image
PARP1 antibody detects PARP1 protein by western blot analysis. Un-treated (-) and treated (+, 30uM cisplatin treatment for 24hr) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody (GTX632388) at a dilution of 1:1000.
GTX632388 IP Image
Immunoprecipitation of PARP protein from 293T whole cell extracts using 5 μg of PARP antibody (GTX632388).
Western blot analysis was performed using PARP antibody (GTX632388).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.