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PARP antibody [HL1365]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805
Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805
Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805
Anti-PARP antibody [HL1365] used in IHC (Paraffin sections) (IHC-P). GTX636805
Anti-PARP antibody [HL1365] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636805
Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805

Cat. No. GTX636805

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1365

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Product Note

This antibody is specific for human PARP1 protein, and it does not cross react with human PARP2 and PARP3 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805

GTX636805 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1365] (GTX636805) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805

GTX636805 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with PARP antibody [HL1365] (GTX636805) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805

GTX636805 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1365] (GTX636805) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [HL1365] used in IHC (Paraffin sections) (IHC-P). GTX636805

GTX636805 IHC-P Image

PARP antibody [HL1365] detects PARP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PARP stained by PARP antibody [HL1365] (GTX636805) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PARP antibody [HL1365] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636805

GTX636805 ICC/IF Image

PARP antibody [HL1365] detects PARP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP stained by PARP antibody [HL1365] (GTX636805) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-PARP antibody [HL1365] used in Western Blot (WB). GTX636805

GTX636805 WB Image

Orthogonal

Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1365] (GTX636805) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations