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PARP antibody [N1N2], N-term

Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839
Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839
Anti-PARP antibody [N1N2], N-term used in ChIP assay (ChIP assay). GTX112839
Anti-PARP antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112839
Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839
Anti-PARP antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112839
Anti-PARP antibody [N1N2], N-term used in ChIP assay (ChIP assay). GTX112839
Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839
Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839
Anti-PARP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112839

Cat No. GTX112839

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , PC-12 , Rat2

Product Note

IP/MS validation was supported by references (PMID:30377408)

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PARP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618945

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839

GTX112839 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000.

Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839

GTX112839 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000 and competitor's antibody (#9542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PARP antibody [N1N2], N-term used in ChIP assay (ChIP assay). GTX112839

GTX112839 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.

Anti-PARP antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112839

GTX112839 ICC/IF Image

PARP antibody [N1N2], N-term detects PARP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP protein stained by PARP antibody [N1N2], N-term (GTX112839) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839

GTX112839 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112839

GTX112839 IP Image

PARP1 antibody [N1N2], N-term immunoprecipitates PARP1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 30 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N1N2], N-term (GTX112839)
5 % SDS-PAGE
The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N1N2], N-term (GTX112839) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-PARP antibody [N1N2], N-term used in ChIP assay (ChIP assay). GTX112839

GTX112839 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839

GTX112839 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N1N2], N-term used in Western Blot (WB). GTX112839

GTX112839 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PARP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112839

GTX112839 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PARP1 antibody [N1N2], N-term(GTX112839) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf