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PARP antibody

Orthogonal
Anti-PARP antibody used in Western Blot (WB). GTX33387
Anti-PARP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33387
Anti-PARP antibody used in IHC (Paraffin sections) (IHC-P). GTX33387
Anti-PARP antibody used in IHC (Paraffin sections) (IHC-P). GTX33387
Anti-PARP antibody used in Western Blot (WB). GTX33387
Anti-PARP antibody used in IHC (Paraffin sections) (IHC-P). GTX33387
Anti-PARP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33387

Cat No. GTX33387

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($369)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP antibody used in Western Blot (WB). GTX33387

GTX33387 WB Image

Orthogonal

WB analysis of Jurkat cell treated by Etoposide (25 uM) at 37ºC for 5 hours using GTX33387 PARP antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-PARP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33387

GTX33387 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33387 PARP antibody.
Blue : DAPI
Dilution : 1:100

Anti-PARP antibody used in IHC (Paraffin sections) (IHC-P). GTX33387

GTX33387 IHC-P Image

IHC-P analysis of rat testis tissue using GTX33387 PARP antibody.
Dilution : 1:100

Anti-PARP antibody used in IHC (Paraffin sections) (IHC-P). GTX33387

GTX33387 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX33387 PARP antibody.
Dilution : 1:100

Anti-PARP antibody used in Western Blot (WB). GTX33387

GTX33387 WB Image

WB analysis of various sample lysates using GTX33387 PARP antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-PARP antibody used in IHC (Paraffin sections) (IHC-P). GTX33387

GTX33387 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX33387 PARP antibody.
Dilution : 1:100

Anti-PARP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33387

GTX33387 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33387 PARP antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369