APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1
Cellular Localization
Nucleus,Nucleolus
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX33387 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX33387 PARP antibody.
Dilution : 1:100
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GTX33387 IHC-P Image
IHC-P analysis of rat testis tissue using GTX33387 PARP antibody.
Dilution : 1:100
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GTX33387 WB Image
WB analysis of Jurkat treated by Etoposide (25 uM) at 37ºC for 5 hours using GTX33387 PARP antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX33387 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX33387 PARP antibody.
Dilution : 1:100
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GTX33387 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX33387 PARP antibody.
Blue : DAPI
Dilution : 1:100
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GTX33387 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX33387 PARP antibody.
Blue : DAPI
Dilution : 1:100
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GTX33387 WB Image
WB analysis of various sample lysates using GTX33387 PARP antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
There are currently no references for PARP antibody (GTX33387). Be the first to share your publications with this product.
REVIEW
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