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PARP (cleaved Asp214) antibody

Anti-PARP (cleaved Asp214) antibody used in Western Blot (WB). GTX132329
Anti-PARP (cleaved Asp214) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132329
Anti-PARP (cleaved Asp214) antibody used in Western Blot (WB). GTX132329

Cat No. GTX132329

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T , HCT116 , HCT116 (30uM cisplatin treatment for 24hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PARP1 (cleaved Asp214). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

poly(ADP-ribose) polymerase 1,ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PARP (cleaved Asp214) antibody used in Western Blot (WB). GTX132329

GTX132329 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP (cleaved Asp214) antibody (GTX132329) diluted at 1:3000.

Anti-PARP (cleaved Asp214) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132329

GTX132329 ICC/IF Image

PARP1 (cleaved Asp214) antibody detects PARP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP1 protein stained by PARP1 (cleaved Asp214) antibody (GTX132329) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PARP (cleaved Asp214) antibody used in Western Blot (WB). GTX132329

GTX132329 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 (cleaved Asp214) antibody (GTX132329) diluted at 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169