GeneTex
United States (US)

PARP (cleaved Gly215) antibody

PARP-cleaved-Gly215-antibody-GTX86935-WB-1_18121411_679.jpg

Cat No. GTX86935

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:1000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

89 kDa. ( Note )

Specificity/Sensitivity

PARP (Cleaved-Gly215) Antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Gly215.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human PARP.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Poly(Adp-Ribose) Polymerase 1,Adprt,Adprt 1,Adprt1,Artd1,Parp,Parp-1,Ppol,Padprt-1,Parp1

Background

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

Database

Research Area

DATA IMAGES
PARP-cleaved-Gly215-antibody-GTX86935-WB-1_18121411_679.jpg

GTX86935 WB Image

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using PARP (Cleaved-Gly215) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339