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PARP (cleaved) antibody (FITC)

Anti-PARP (cleaved) antibody (FITC) used in Flow cytometry (FACS). GTX24831

Cat. No. GTX24831

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IHC

Reactivity

 Human, Mouse, Bovine
Package
500 μl ($399)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Product Note

This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized peptide corresponding to N-terminus of cleavage site (214/215) of human PARP.

Purification

Purified by affinity chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP (cleaved) antibody (FITC) used in Flow cytometry (FACS). GTX24831

GTX24831 FACS Image

FACS analysis of permeabilized, fixed cells (10 μL/10⁶) with apoptosis induction using GTX24831 PARP (cleaved) antibody (FITC). (left-hand figure)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399