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PARP (cleaved) antibody [GT1181]

Recombinant antibody KO/KD Orthogonal
Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943
Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943
Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943
Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943
Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943

Cat. No. GTX00943

Reference 3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1181

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Pig
Package
100 μl ($399)
See all PARP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Observed MW

~25 kDa. The observed M.W. is based on the following publication. PMID: 31045193

Product Note

This antibody is specific for cleaved PARP (~25kDa). It does not recognize full-length (~116kDa) and the large fragment (~89 kDa).

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Cleaved PARP.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885207

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP1 , PPOL , pADPRT1 , poly(ADPribose) polymerase 1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943

GTX00943 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PARP (cleaved) antibody [GT1181] (GTX00943) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943

GTX00943 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PARP (cleaved) antibody [GT1181] (GTX00943) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943

GTX00943 WB Image

KO/KD

WB analysis of wild-type (WT) and PARP (cleaved) knockout (KO) HeLa cell extracts using GTX00943 PARP (cleaved) antibody [GT1181].
Dilution : 1:1000
Laoding : 25μg

Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943

GTX00943 WB Image

Orthogonal

WB analysis of Jurkat cell extracts with/without Staurosporine (1μM, RT, 3 hrs) treatment using GTX00943 PARP (cleaved) antibody [GT1181].
Dilution : 1:1000
Loading : 25μg

Anti-PARP (cleaved) antibody [GT1181] used in Western Blot (WB). GTX00943

GTX00943 WB Image

Orthogonal

WB analysis of mouse spleen tissue and NIH-3T3 cell lysate using GTX00943 PARP (cleaved) antibody [GT1181].
Lane 1 : mouse spleen tissue lysate
Lane 2 : NIH-3T3 whole cell lysate
Dilution : 1:1000
Loading : 25μg

REFERENCE

Application Reference

Jung W et al. Front Vet Sci 2021; 8 : 641916 Expression of Caspases in the Pig Endometrium Throughout the Estrous Cycle and at the Maternal-Conceptus Interface During Pregnancy and Regulation by Steroid Hormones and Cytokines.
Application : IHC-P
Reactivity : Pig
Meng X et al. Front Oncol 2020; 10 : 217 Apatinib Inhibits Cell Proliferation and Induces Autophagy in Human Papillary Thyroid Carcinoma via the PI3K/Akt/mTOR Signaling Pathway.
Application : WB
Reactivity : Human
Wang YY et al. ACS Omega 2020; 5 (12) : 7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399