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PARP (p116/p85) antibody [E78], C-term

Anti-PARP (p116/p85) antibody [E78], C-term used in Western Blot (WB). GTX61031
Anti-PARP (p116/p85) antibody [E78], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX61031

Cat. No. GTX61031

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 E78

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PARP products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1,000
Optimal working dilution for a specific application should be determined by the investigator.

Calculated MW

113 kDa. ( Note )

Observed MW

116 | 85 kDa.

Product Note

The antibody should recognize both pro-form and p85 cleaved-form of PARP-1.

PROPERTIES

Form

Liquid

Buffer

50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to residues near to C-terminal residues of human PARP-1 was used as immunogen.

Purification

Cell Supernatant

Conjugation

Unconjugated

RRID

AB_10619192

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.

TARGET

Synonyms

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PARP (p116/p85) antibody [E78], C-term used in Western Blot (WB). GTX61031

GTX61031 WB Image

A. Western blot analysis of (1) Jurkat cells and (2) Jurkat + Staurosporine using anti-PARP-1 (p116/p85) RabMAb (catalog #GTX61031) 1:1000 dilution.

Anti-PARP (p116/p85) antibody [E78], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX61031

GTX61031 ICC/IF Image

The data was published in the journal PLoS One in 2015. PMID: 26218895

REFERENCE

Application Reference

Olah G et al. PLoS One 2015; 10 (7) : e0134227 Differentiation-Associated Downregulation of Poly(ADP-Ribose) Polymerase-1 Expression in Myoblasts Serves to Increase Their Resistance to Oxidative Stress.
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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399