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PARP14 antibody

Anti-PARP14 antibody used in Western Blot (WB). GTX131920
Anti-PARP14 antibody used in Immunoprecipitation (IP). GTX131920

Cat No. GTX131920

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

203 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PARP14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

poly(ADP-ribose) polymerase family member 14,ARTD8,BAL2,PARP-14,pART8

Cellular Localization

Nucleus , Cytoplasm

Background

Poly(ADP-ribosyl)ation is an immediate DNA damage-dependent posttranslational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP14 belongs to the superfamily of enzymes that perform this modification (Ame et al., 2004 [PubMed 15273990]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-PARP14 antibody used in Western Blot (WB). GTX131920

GTX131920 WB Image

PARP14 antibody detects PARP14 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP14 antibody (GTX131920) diluted at a dilution of 1:1000.

Anti-PARP14 antibody used in Immunoprecipitation (IP). GTX131920

GTX131920 IP Image

Immunoprecipitation of PARP14 protein from SK-N-SH whole cell extracts using 5 μg of PARP14 antibody (GTX131920).
Western blot analysis was performed using PARP14 antibody (GTX131920).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169