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PARP3 antibody

Anti-PARP3 antibody used in Western Blot (WB). GTX103147
Anti-PARP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103147
Anti-PARP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103147
Anti-PARP3 antibody used in Western Blot (WB). GTX103147
Anti-PARP3 antibody used in Western Blot (WB). GTX103147

Cat No. GTX103147

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431 , HeLa , A375 , mouse heart , PC-12
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

poly(ADP-ribose) polymerase family member 3 , ADPRT3 , ADPRTL2 , ADPRTL3 , ARTD3 , IRT1 , PADPRT-3

Cellular Localization

Nucleus , Centrosome , centriole

Background

The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PARP3 antibody used in Western Blot (WB). GTX103147

GTX103147 WB Image

PARP3 antibody detects PARP3 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PARP3 antibody (GTX103147) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103147

GTX103147 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PARP3(GTX103147) antibody at 1:500 dilution.

Anti-PARP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103147

GTX103147 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using PARP3(GTX103147) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PARP3 antibody used in Western Blot (WB). GTX103147

GTX103147 WB Image

Sample (50 µg of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX103147 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP3 antibody used in Western Blot (WB). GTX103147

GTX103147 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP3 antibody (GTX103147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169