GeneTex
United States (US)

PARP3 antibody

GTX103147_39974_WB_R_19011506_950.jpg
GTX103147_39974_WB_19011506_461.jpg
GTX103147_39974_IFA_19011506_266.jpg
GTX103147_IHC_19011506_780.jpg
GTX103147_39974_WB_M_Heart_19011506_229.jpg

Cat No. GTX103147

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431 , HeLa , A375 , mouse heart , PC-12
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ADPRT3 antibody, ADPRTL2 antibody, ADPRTL3 antibody, IRT1 antibody, PADPRT-3 antibody, PARP3 antibody, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 3 antibody, NAD(+) ADP-ribosyltransferase 3 antibody, poly[ADP-ribose] synthase 3 antibody, NAD+ ADP-ribosyltransferase 3 antibody, poly(ADP-ribose) synthetase-3 antibody, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 2 antibody, poly[ADP-ribose] synthetase 3 antibody, poly [ADP-ribose] polymerase 3 antibody, ADPRT-3 antibody, poly (ADP-ribose) polymerase family, member 3 antibody

Cellular Localization

Nucleus , Centrosome , centriole

Background

The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX103147_39974_WB_R_19011506_950.jpg

GTX103147 WB Image

PARP3 antibody detects PARP3 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PARP3 antibody (GTX103147) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103147_39974_WB_19011506_461.jpg

GTX103147 WB Image

PARP3 antibody detects PARP3 protein by western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg A375 whole cell lysate/extract
7.5% SDS-PAGE
PARP3 antibody (GTX103147) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103147_39974_IFA_19011506_266.jpg

GTX103147 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PARP3(GTX103147) antibody at 1:500 dilution.

GTX103147_IHC_19011506_780.jpg

GTX103147 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using PARP3(GTX103147) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103147_39974_WB_M_Heart_19011506_229.jpg

GTX103147 WB Image

Sample (50 µg of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX103147 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169