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PATJ antibody [N1], N-term

Anti-PATJ antibody [N1], N-term used in Western Blot (WB). GTX104753
Anti-PATJ antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104753

Cat No. GTX104753

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

196 kDa. ( Note )

Positive Control

Mouse bladder

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PATJ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PATJ crumbs cell polarity complex component , Cipp , INADL , InaD-like , hINADL

Cellular Localization

Membrane , Cell junction , tight junction , Apical cell membrane

Background

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. [provided by RefSeq]

Database

DATA IMAGES
Anti-PATJ antibody [N1], N-term used in Western Blot (WB). GTX104753

GTX104753 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

Anti-PATJ antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104753

GTX104753 IHC-P Image

PATJ antibody [N1], N-term detects PATJ protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169