GeneTex
United States (US)

PATJ antibody [N1], N-term

GTX104753_39666_20151112_WB_M_bladder_19011506_662.jpg
GTX104753_39666_20151123_IHC-P_19011506_382.jpg

Cat No. GTX104753

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

196 kDa. ( Note )

Positive Control

Mouse bladder

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PATJ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cipp antibody, FLJ26982 antibody, InaD-like antibody, PATJ antibody, hINADL antibody, INADL antibody, protein associated to tight junctions antibody, PDZ domain protein antibody, inaD-like protein antibody, channel-interacting PDZ domain protein antibody, PALS1-associated tight junction protein antibody, inactivation no after-potential D-like protein antibody, InaD-like (Drosophila) antibody

Cellular Localization

Membrane , Cell junction , tight junction , Apical cell membrane

Background

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. [provided by RefSeq]

Database

DATA IMAGES
GTX104753_39666_20151112_WB_M_bladder_19011506_662.jpg

GTX104753 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

GTX104753_39666_20151123_IHC-P_19011506_382.jpg

GTX104753 IHC-P Image

PATJ antibody [N1], N-term detects PATJ protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169